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Journal: iScience
Article Title: Regulatory changes underlying the evolution of skylight navigation
doi: 10.1016/j.isci.2026.116313
Figure Lengend Snippet: Absence of Homothorax from DRA ommatidia beyond higher diptera (A and A′) Homothorax ( Hth, yellow) immunostaining in the adult brain of a honeybee worker ( Apis mellifera ) labels distinct neuronal population. (A'' and A''') Pupal retina (13-day-old worker) showing the most dorsal ommatidia. The boxed region marks the ROI shown at higher magnification. Hth is clearly localized to the pigment rim and bristle cells but absent from ommatidia adjacent to the rim. (B and B′) Anti-Hth immunostaining in the larval brain of the monarch butterfly ( Danaus plexippus ) labels specific cells distinct from Bar-positive (cyan) cell populations. (B'' and B'''') Dorsal pupal retina (4-day-old) labeled with Anti-Bar (outer photoreceptors) and Anti-Spalt (Sal ; inner photoreceptors). Anti-Hth signal appears only as background autofluorescence, with no specific labeling. (C and C′) Whole-mounted fifth instar larval brain of Vanessa cardui stained with monarch Anti-Hth antibody (yellow) and Anti-Bar (cyan) antibody, confirming non-overlapping expression. The Dp Hth antibody cross-reacts and functions in Vanessa cardui. (C'' and C'''') Control staining in a 4-day-old V. cardui pupal retina shows strong hth expression in the pigment rim but not in adjacent ommatidia. (D and D′) Anti-Hth immunostaining in the pupal brain of Aedes aegypti labels distinct neuronal sets. (D'' and D'''') Pupal retina (1-day-old) labeled with Aa Op8 (marking all R7 photoreceptors except those in the dorsal region). Hth localization is confined to the pigment rim and pigment cells, with no detectable signal in photoreceptors. In all brain samples, DAPI (magenta) was used to label all nuclei. In all retinal close-up images, “P” denotes the pigment rim, with the dashed line outlining the area adjacent to the photoreceptors. Per species: N = 3 (brain) and N = 5 (retina). Scale bars, 50 μm (brain) and 20 μm (retina).
Article Snippet:
Techniques: Immunostaining, Labeling, Staining, Expressing, Control